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Hydrologie du bassin de Marennes- Oléron. Analyse de la base de données "RAZLEC" 1977- 1995 ArchiMer
Faury, Nicole; Razet, Daniel; Soletchnik, Patrick; Goulletquer, Philippe; Ratiskol, Jacqueline; Garnier, Jacqueline.
An hydrobiological monitoring survey has been carried out in the Bay of Marennes-Oléron since 1977. Five stations, distributed over the Bay, were sam pied twice a month. Spatial and temporal trends of the physical characteristics (i.e., seawater, temperature, salinity, dissolved oxygen and suspended matter), nutrients (azote, nitrite, nitrate, silicate) and organic variables (chlorophyll a and pheopigments) were assessed by using a statistical time-series analysis mode\. It was used to distinguish seasonal variability from long term trends. Pluviometric data as weil as the Charente River fluxes were similary analyzed by the model. The seasonal variability showed an homogeneous pattern over the five stations for all parameters, but the site-dependent...
Tipo: Text Palavras-chave: Shellfish farming; Overall trends; Seawater seasonal variability; Marennes Oleron Bay; Hydrology; Conchyliculture; Tendances; Variations saisonnières; Marennes Oléron; Hydrologie.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/rapport-6379.pdf
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HYDROMAR. Variabilité spatiale de la qualité hydrologique des eaux côtières alimentant les marais salés endigués et les claires de Charente-Maritime. 1. Travaux de l'année 2001 ArchiMer
Hussenot, Jerome; Faury, Nicole; Fillon, Alain; Galvan, Audrey.
Un protocole d 'échantillonnage et de mesure de la qualité des eaux marines pénétrant dans les marais salés endigués et claires ostréicoles du littoral de la Charente-Maritime a été mis en place en 2001 dans le cadre du projet IFREMER /Poitou-Charentes sur la «gestion durable des activités anthropiques en marais et zone littorale». Ce réseau d 'observation sera poursuivi chaque année tout au long de la période du contrat de plan, lors d'une pleine mer de vives eaux d 'automne (Novembre ou Décembre), en recherchant des conditions de marée théoriquement similaires (coefficient et heures de pleine mer proches chaque année d 'observation). La première campagne a montré l'importance de l 'eau douce dans les apports de matière dissoute minérale (sels nutritifs...
Tipo: Text Palavras-chave: Qualité eaux côtières; Variabilité spatiale; Marais; Claires ostréicoles; Charente-Maritime.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00199/31017/29422.pdf
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Identification of genes from flat oyster Ostrea edulis as suitable housekeeping genes for quantitative real time PCR ArchiMer
Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan.
Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their...
Tipo: Text Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf
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Identification of the autophagy pathway in a mollusk bivalve, Crassostrea gigas ArchiMer
Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin.
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members...
Tipo: Text Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf
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Immune-suppression by OsHV-1 viral infection causes fatal bacteraemia in Pacific oysters ArchiMer
De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume.
Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial...
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Ano: 2018 URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf
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Impact on non target organisms of anti-marine wood-borer treatments. Evaluation of stress imposed by treatments on organisms form test sites - Final Report ArchiMer
Goulletquer, Philippe; Geairon, Philippe; Faury, Nicole; Razet, Daniel; Soletchnik, Patrick; Adessi, L.; Gras, Paul.
Experiments were canied out to assess the copper-chrome-arsenic (CCA) trealment effeets on the Blue mussel Mytilus edulis physiology. Experimental design included two complementary approaches: 1) scope for growth esrimates based upon the energy balance between respiration energy loss and energy gained from feeding processes. 2) a biological early waming system for valve behavior monitoring under CCA exposures. Various temperature range (seasonal effeet). physiological status (mature & immature mussels) as weil as CCA concentrations (from 0 to 48kg/m3) and rime exposure were tested. Significant monality rates for the experimental mussel populations were recorded in alI experimental modalities but the 2kg/m3 CCA treatment concentration. Overall results...
Tipo: Text Palavras-chave: Bivalvia; Mussel; Mytilus edulis; Copper-Chrome-Arsenic (CCA); Treatment; Feeding; Absorption.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/00077/18795/16372.pdf
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Impact on non target organisms of anti-marine wood-borer treatments. Evaluation of stress imposed by treatments on organisms from test sites ArchiMer
Goulletquer, Philippe; Geairon, Philippe; Faury, Nicole; Razet, Daniel; Soletchnik, Patrick; Gras, Paul; Adessi, L..
In European waters, significant economic damages to wooden structures are caused by bivalves molluscs and crustaceans. Treatments like copper-chrome-arsenic mixtures (CCA) were designed to prevent settlement of these wood borer species. However, limited and conflicting evidence regarding the toxicity to non-target organisms are available in the literature. This report aims to provide insights regarding the CCA leachates' toxicity to an important aquaculture species, the blue mussel Mytilus edulis for which much information on normal and stressed physiology is available for purposes of comparisons. The mussels are usually considered as a particularly suitable species for biological monitoring (Smaal and Widdows, 1994). To address the CCA leachate issue, two...
Tipo: Text Palavras-chave: Bivalvia; Mussel; Mytilus edulis; Copper-chrome-arsenic (CCA); Treatment; Feeding; Absorption.
Ano: 1996 URL: http://archimer.ifremer.fr/doc/00043/15437/12788.pdf
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In situ localization and tissue distribution of ostreid herpesvirus 1 proteins in infected Pacific oyster, Crassostrea gigas ArchiMer
Martenot, Claire; Segarra, Amelie; Baillon, Laury; Faury, Nicole; Houssin, Maryline; Renault, Tristan.
Immunohistochemistry (IHC) assays were conducted on paraffin sections from experimentally infected spat and unchallenged spat produced in hatchery to determine the tissue distribution of three viral proteins within the Pacific oyster, Crassostrea gigas. Polyclonal antibodies were produced from recombinant proteins corresponding to two putative membrane proteins and one putative apoptosis inhibitor encoded by ORF 25, 72, and 87, respectively. Results were then compared to those obtained by in situ hybridization performed on the same individuals, and showed a substantial agreement according to Landis and Koch numeric scale. Positive signals were mainly observed in connective tissue of gills, mantle, adductor muscle, heart, digestive gland, labial palps, and...
Tipo: Text Palavras-chave: OsHV-1; Viral proteins; Crassostrea gigas.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00326/43697/43124.pdf
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Influence de la mise en culture de l'Ilot des Tannes sur la qualité bactériologique des eaux du chenal de Brouage - Campagnes 1985-1986 ArchiMer
Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline.
Les agriculteurs aménageant quelques centaines d'hectares du marais doux de Hiers Brouage (mise à plat, drainage) afin de le mettre en culture, il était important de pouvoir mesurer l'impact que peuvent avoir les rejets de drainage-pompage dans le milieu marin par l'intermédiaire des chenaux ostréicoles. Ceci afin d'éviter d'éventuels conflits entre groupes socio-professionnels (agriculteurs et conchyliculteurs) en proposant des mesures et de solutions. Un suivi est donc effectué à la demande des administrations, en collaboration avec l'INRA et le CEMAGREF, lesquels assurent le suivi de la lagune de décantation des eaux de drainage. L'IFREMER prenant en compte le milieu récepteur (chenaux du Grand Garçon et de Brouage). A raison de cinq sériées de...
Tipo: Text Palavras-chave: Qualité des eaux; Agriculture; Rejets; Marais maritimes; Chenal de Brouage; Charente-Maritime.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00087/19850/17500.pdf
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Influence de la mise en culture de l'Ilot des Tannes sur la qualité bactériologique des eaux du chenal de Brouage - Campagnes 1986-1987 ArchiMer
Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline.
L'étude entreprise en 1985 sur la qualité des eaux de rejet de la zone cultivée de l'Ilot des Tannes, dans le marais de Moeze, examine maintenant les résultats de la deuxième année de culture intensive. Rappelons que cette étude a pour but d'apprécier la qualité physico-chimique des eaux de ruissellement et le drainage arrivant dans le milieu ostréicole par l'intermédiaire du Chenal de Brouage. Les compagnes des deux années précédentes après un point zéro et une première année de mise en culture avaient fait apparaître, outre la présence d'insecticides rémanents, une dominance qualitative et quantitative des herbicides aussi bien dans l'eau que dans la matière vivante. Chaque année, des difficultés techniques différentes limitent l'information recueillie,...
Tipo: Text Palavras-chave: Qualité des eaux; Marais Maritime; Agriculture; Rejets agricoles; Chenal de Brouage; Charente-Maritime.
Ano: 1988 URL: http://archimer.ifremer.fr/doc/00087/19849/17499.pdf
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Influence de la mise en culture de l'Ilot des Tannes sur la qualité des eaux du chenal de Brouage - Campagnes 1984-1985 ArchiMer
Coeurdacier, Jean-luc; Faury, Nicole; Ratiskol, Jacqueline.
Les agriculteurs envisagent ou ont commencé d'aménager certains marais doux à des fins de culture. Si ces projets se réalisent, 500ha seront aménagés à terme (2 à 5 ans) dans le marais de Hiers Brouage qui en totalise 10 500. Les aménagements comportent : un isolement des ilots, une mise à plat, un assainissement par drainage et pompage de l'eau avec rejet direct ou indirect dans le milieu marin. Les administrations conscientes des risques de conflit entre agriculteurs d'une part, ostréiculteurs et associations de protection de la nature d'autre part ont souhaité qu'un suivi soit effectué sur l'Ilot des Tannes (115 ha) pris comme test. Le principe d'une "lagune" a été retenu afin d'arrêter les particules fines et provoquer une certaine stabulation des eaux...
Tipo: Text Palavras-chave: Qualité des eaux; Agriculture; Conchyliculture; Rejets agricoles; Chenal de Brouage; Charente-Maritime.
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00087/19851/17501.pdf
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Influence de l'apport de la Charente sur la qualité bactériologique des eaux de l'estuaire - Campagnes 1988 et 1989 ArchiMer
Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline.
Le bassin de Marennes Oléron, comme toutes les zones conchylicoles, est susceptible d'être touché par les apports pollués. Dans ce domaine, le vecteur potentiel que constitue la Charente méritait d'être étudié plus particulièrement, la qualité bactériologique des eaux pouvant affecter par la suite la salubrité des coquillages. L'étude précédente (Masson et al. 1988) a fait apparaître que la pollution bactérienne apportée par la Charente (particulièrement en étiage) sédimentait rapidement, la quantité de germes devenant très faible à la sortie de l'estuaire. Toutefois, comme l'enjeu économique est important (perspectives européennes) il était nécessaire de confirmer ce résultat avec un système de suivi identique à celui utilisé en 1987. De plus, un certain...
Tipo: Text Palavras-chave: Bactériologie; Qualité eaux; Salubrité coquillages; Pollution bactérienne; Estuaire; Charente; Charente Maritime Dep..
Ano: 1990 URL: http://archimer.ifremer.fr/doc/00087/19827/17469.pdf
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Le risque trophique : caractéristiques trophique et hétérogénéité des ressources alimentaires en lien avec les mortalités ostréicoles ArchiMer
Ropert, Michel; Lefebvre, Sebastien; Soletchnik, Patrick; Blin, J. L.; Faury, Nicole; Struski, Caroline.
The "trophic risk" has been demonstrated in the bioenergetic model developed in vivo and based on various diet conditions similar to those of different types of oyster culture basins (eutrophic, heterotrophic and oligotrophic) [22, 23]. The trophic resource – mortality relationship differs from one basin to another, according to the quantity of this resource. Multy-varied analysis (ACP) conducted on a total of 11 oyster culture sites and handled in a database matching mortality and environmental data, show that the trophic indicator "chlorophyll a" (often coupled with temperature) is involved in a positive correlation with the mortality of both age classes (1 and 2 years old). The stock transfer from the Bay of Veys to the west coast of Cotentin in spring...
Tipo: Text Palavras-chave: MOREST; Crassostrea gigas; Physiologie; Phéopigments; Ressources trophiques; Modèle bioénergétique; Mortalités; Huîtres.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/acte-3410.pdf
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Les analyses biochimiques de proteines, lipides et glucides sur l'huître creuse Crassostrea gigas ArchiMer
Razet, Daniel; Faury, Nicole; Soletchnik, Patrick; Geairon, Philippe.
Our objectives were to: -update analytical methods of proteins, lipids and carbohydrates in oyster flesh; and -attempt to better understand the high variability in the results of biochemical analyses and their ability to explain the composition of organic matter (dry weight of oyster flesh).
Tipo: Text Palavras-chave: Methodes analyses; Biochimie; Crassostrea gigas.
Ano: 1997 URL: http://archimer.ifremer.fr/doc/1997/acte-3256.pdf
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Long dsRNAs promote an anti-viral response in Pacific oyster hampering ostreid herpesvirus 1 replication ArchiMer
Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud.
Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters...
Tipo: Text Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf
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Métabolisme de l'huître Crassostrea gigas stockée dans des dégorgeoirs conchylicoles, à différentes densités et saisons ArchiMer
Bougrier, Serge; Masson, Daniel; Geairon, Philippe; Fouche, Dominique; Leroy, Anne; Faury, Nicole; Ratiskol, Jacqueline.
One of the problems faced by oyster farmers in France is the immediate or delayed mortality of oysters in the storage tanks prior to shipping. This may be due to overstocking and/or improper water management. To investigate the problem, experiments were performed in commercial storage tanks stocked with oysters at different densities. The aim of this study was to provide some basic experimental data and guidelines for better handling of oysters prior to shipping
Tipo: Text Palavras-chave: Physiology; Oyster farmers; Crassostrea gigas; Oysters; Storage tanks; Physiologie; Ostréiculture; Dégorgeoirs; Crassostrea gigas; Huîtres; Bassin stockage.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-3047.pdf
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Molecular responses of Ostrea edulis haemocytes to an in vitro infection with Bonamia ostreae ArchiMer
Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan.
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in...
Tipo: Text Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf
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Mortalité de l'huître Crassostrea gigas dans le bassin de Marennes-Oléron: étude de la variabilité spatiale de son environnement et de sa biologie par un système d'informations géographiques (SIG) ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Razet, Daniel; Geairon, Philippe; Goulletquer, Philippe.
The summer mortality patterns was studied in 1996 on the Ronce-les-Bains oyster bank (175 ha), located on the southern part of the Bay Marennes-Oleron (Atlantic coastline). Studies on growth, sexual maturation, survival rates and environmental variables were compared at fifteen experimental sites (on- and off-bottom culture). After 7 months, cumulative mortality reached 8-19 and 23-33% for off-bottom and on-bottom culture, respectively which is significantly higher for the latter case. A seasonal mortality trend was noted, with a significant increase related to air temperature greater than 20 degrees C and with a significant pre-spawning glycogen catabolism. Based on soft tissue flesh growth results from both types of culture, several empirical models were...
Tipo: Text Palavras-chave: Atlantic Ocean; Crassostrea gigas; Mortality; Growth rates; Océan Atlantique; Crassostrea gigas; Mortalité; Croissance.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/1999/publication-862.pdf
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Mortalité de l'huître creuse C. gigas dans le Bassin de Marennes-Oléron. Résultats obtenus sur le site atelier de Ronce-Perquis en 1997-1998 ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Razet, Daniel; Geairon, Philippe; Robert, Stephane; Goulletquer, Philippe.
The cupped oyster, Crassostrea gigas was first introduced in France in 1967 [13]. On the Atlantic coast, the Marennes Oléron Bay (Figure 1) is the first European oyster culture basin with a cupped oyster (Crassostrea gigas) stock of 110,000 tons and an annual output of 30,000 to 40,000 tons. The Ronce oyster bank, located at the south of the bay (Figure 1), is made of 1,600 concessions spread out over 175 ha. It produces 8,000 to 10,000 tons of cupped oysters each year. In Japan, Crassostrea gigas has been cultivated for more than 1,000 years (Farley, 1992). Since the beginning of the 1960's, oyster mortality has multiplied throughout the world (Imaï et al., 1965; Sinderman, 1976; Mackin, 1961; Beattie et al, 1980). In the 60's and 70's, Japan and the west...
Tipo: Text Palavras-chave: Crassostrea gigas; Physiologie; Pontes; Maturation; Croissance; Marennes Oléron; Huîtres; Mortalités.
Ano: 1998 URL: http://archimer.ifremer.fr/doc/1998/rapport-3380.pdf
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Mortalité et croissance de l'huître creuse C. gigas dans le bassin de Marennes Oléron. Résultats obtenus sur le site atelier de Ronce-Perquis en 1997-1999 ArchiMer
Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Razet, Daniel; Geairon, Philippe; Robert, Stephane; Goulletquer, Philippe; Taillade, Sylvie.
The 1999 monitoring of the C. gigas mortality rates showed a 32 to 40% loss for the on-bottom rearings located on the Ronce-Perquis oyster bank. In 1997, the averaged mortality rate varied between 23 and 29% without significant site effect. These mortality rates were ranging from 17 to 32% in 1998 with significant limited mortality rate on site 4 (17%). However, those rates were underestimated by 7 to 8%, based on our 1999 study, Actually, the spatial distribution for these rates on 2 experimental sites divided into 25 subunits demonstrated a large variability ranging from 17-37% and 22-56% for sites 1 and 2, respectively. In spite this large variability, the mortality rates results were larger on the muddy bottom site. In contrast to site 1, the oysters...
Tipo: Text Palavras-chave: Bay of Marennes Oleron; Crassostrea gigas; Reproductive effort; Biochemical composition; Growth; Sampling strategy; Mortality rates; Bassin de Marennes Oléron; Crassostrea gigas; Effort de reproduction; Composition biochimique; Croissance; Méthodologie d'estimation; Taux de mortalité.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/rapport-1641.pdf
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