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Registros recuperados: 120 | |
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Faury, Nicole; Razet, Daniel; Soletchnik, Patrick; Goulletquer, Philippe; Ratiskol, Jacqueline; Garnier, Jacqueline. |
An hydrobiological monitoring survey has been carried out in the Bay of Marennes-Oléron since 1977. Five stations, distributed over the Bay, were sam pied twice a month. Spatial and temporal trends of the physical characteristics (i.e., seawater, temperature, salinity, dissolved oxygen and suspended matter), nutrients (azote, nitrite, nitrate, silicate) and organic variables (chlorophyll a and pheopigments) were assessed by using a statistical time-series analysis mode\. It was used to distinguish seasonal variability from long term trends. Pluviometric data as weil as the Charente River fluxes were similary analyzed by the model. The seasonal variability showed an homogeneous pattern over the five stations for all parameters, but the site-dependent... |
Tipo: Text |
Palavras-chave: Shellfish farming; Overall trends; Seawater seasonal variability; Marennes Oleron Bay; Hydrology; Conchyliculture; Tendances; Variations saisonnières; Marennes Oléron; Hydrologie. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/rapport-6379.pdf |
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Hussenot, Jerome; Faury, Nicole; Fillon, Alain; Galvan, Audrey. |
Un protocole d 'échantillonnage et de mesure de la qualité des eaux marines pénétrant dans les marais salés endigués et claires ostréicoles du littoral de la Charente-Maritime a été mis en place en 2001 dans le cadre du projet IFREMER /Poitou-Charentes sur la «gestion durable des activités anthropiques en marais et zone littorale». Ce réseau d 'observation sera poursuivi chaque année tout au long de la période du contrat de plan, lors d'une pleine mer de vives eaux d 'automne (Novembre ou Décembre), en recherchant des conditions de marée théoriquement similaires (coefficient et heures de pleine mer proches chaque année d 'observation). La première campagne a montré l'importance de l 'eau douce dans les apports de matière dissoute minérale (sels nutritifs... |
Tipo: Text |
Palavras-chave: Qualité eaux côtières; Variabilité spatiale; Marais; Claires ostréicoles; Charente-Maritime. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/00199/31017/29422.pdf |
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Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Renault, Tristan. |
Bonamia ostreae is an intrahaemocytic protozoan affecting Ostrea edulis. The parasite multiplies within haemocytes without being degraded and involves changes in cellular activities. Studies aiming at better understanding host response to a pathogen at the transcriptome levels are frequently based on the use of real time PCR assays, which require some reference genes. However, very few sequence data is available for O. edulis in public databases. Subtracted cDNA libraries were constructed from the O. edulis haemocytes in order to identify genes involved in host reactions against the parasite and quantitative real time PCR assays were developed to study expression of these genes. In this context, identification of reference genes and study of their... |
Tipo: Text |
Palavras-chave: Real time PCR; Housekeeping genes; Haemocytes; Ostrea edulis; Bonamia ostreae. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00018/12880/9829.pdf |
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Picot, Sandy; Faury, Nicole; Arzul, Isabelle; Chollet, Bruno; Renault, Tristan; Morga, Benjamin. |
The Pacific oyster, Crassostrea gigas, is a mollusk bivalve commercially important as a food source. Pacific oysters are subjected to stress and diseases during culture. The autophagy pathway is involved in numerous cellular processes, including responses to starvation, cell death, and microorganism elimination. Autophagy also exists in C. gigas, and plays a role in the immune response against infections. Although this process is well-documented and conserved in most animals, it is still poorly understood in mollusks. To date, no study has provided a complete overview of the molecular mechanism of autophagy in mollusk bivalves. In this study, human and yeast ATG protein sequences and public databases (Uniprot and NCBI) were used to identify protein members... |
Tipo: Text |
Palavras-chave: Autophagy; Autophagy related; Crassostrea gigas; Data mining; Immunohistochemistry; Real-time PCR; Western blot. |
Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71785/70270.pdf |
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De Lorgeril, Julien; Lucasson, Aude; Petton, Bruno; Toulza, Eve; Montagnani, Caroline; Clerissi, Camille; Vidal-dupiol, Jeremie; Chaparro, Cristian; Galinier, Richard; Escoubas, Jean Michel; Haffner, Philippe; Degremont, Lionel; Charriere, Guillaume; Lafont, Maxime; Delort, Abigail; Vergnes, Agnes; Chiarello, Marlene; Faury, Nicole; Rubio, Tristan; Leroy, Marc; Perignon, Adeline; Regler, Denis; Morga, Benjamin; Alunno-bruscia, Marianne; Boudry, Pierre; Le Roux, Frederique; Destoumieux-garzon, Delphine; Gueguen, Yannick; Mitta, Guillaume. |
Infectious diseases are mostly explored using reductionist approaches despite repeated evidence showing them to be strongly in fluenced by numerous interacting host and environmental factors. Many diseases with a complex aetiology therefore remain misunderstood. By developing a holistic approach to tackle the complexity of interactions, we decipher the complex intra-host interactions underlying Pacific oyster mortality syndrome affecting juveniles of Crassostrea gigas, the main oyster species exploited worldwide. Using experimental infections reproducing the natural route of infection and combining thorough molecular analyses of oyster families with contrasted susceptibilities, we demonstrate that the disease is caused by multiple infection with an initial... |
Tipo: Text |
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Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00461/57234/59228.pdf |
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Goulletquer, Philippe; Geairon, Philippe; Faury, Nicole; Razet, Daniel; Soletchnik, Patrick; Gras, Paul; Adessi, L.. |
In European waters, significant economic damages to wooden structures are caused by bivalves molluscs and crustaceans. Treatments like copper-chrome-arsenic mixtures (CCA) were designed to prevent settlement of these wood borer species. However, limited and conflicting evidence regarding the toxicity to non-target organisms are available in the literature. This report aims to provide insights regarding the CCA leachates' toxicity to an important aquaculture species, the blue mussel Mytilus edulis for which much information on normal and stressed physiology is available for purposes of comparisons. The mussels are usually considered as a particularly suitable species for biological monitoring (Smaal and Widdows, 1994). To address the CCA leachate issue, two... |
Tipo: Text |
Palavras-chave: Bivalvia; Mussel; Mytilus edulis; Copper-chrome-arsenic (CCA); Treatment; Feeding; Absorption. |
Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00043/15437/12788.pdf |
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Martenot, Claire; Segarra, Amelie; Baillon, Laury; Faury, Nicole; Houssin, Maryline; Renault, Tristan. |
Immunohistochemistry (IHC) assays were conducted on paraffin sections from experimentally infected spat and unchallenged spat produced in hatchery to determine the tissue distribution of three viral proteins within the Pacific oyster, Crassostrea gigas. Polyclonal antibodies were produced from recombinant proteins corresponding to two putative membrane proteins and one putative apoptosis inhibitor encoded by ORF 25, 72, and 87, respectively. Results were then compared to those obtained by in situ hybridization performed on the same individuals, and showed a substantial agreement according to Landis and Koch numeric scale. Positive signals were mainly observed in connective tissue of gills, mantle, adductor muscle, heart, digestive gland, labial palps, and... |
Tipo: Text |
Palavras-chave: OsHV-1; Viral proteins; Crassostrea gigas. |
Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00326/43697/43124.pdf |
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Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline. |
Les agriculteurs aménageant quelques centaines d'hectares du marais doux de Hiers Brouage (mise à plat, drainage) afin de le mettre en culture, il était important de pouvoir mesurer l'impact que peuvent avoir les rejets de drainage-pompage dans le milieu marin par l'intermédiaire des chenaux ostréicoles. Ceci afin d'éviter d'éventuels conflits entre groupes socio-professionnels (agriculteurs et conchyliculteurs) en proposant des mesures et de solutions. Un suivi est donc effectué à la demande des administrations, en collaboration avec l'INRA et le CEMAGREF, lesquels assurent le suivi de la lagune de décantation des eaux de drainage. L'IFREMER prenant en compte le milieu récepteur (chenaux du Grand Garçon et de Brouage). A raison de cinq sériées de... |
Tipo: Text |
Palavras-chave: Qualité des eaux; Agriculture; Rejets; Marais maritimes; Chenal de Brouage; Charente-Maritime. |
Ano: 1987 |
URL: http://archimer.ifremer.fr/doc/00087/19850/17500.pdf |
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Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline. |
L'étude entreprise en 1985 sur la qualité des eaux de rejet de la zone cultivée de l'Ilot des Tannes, dans le marais de Moeze, examine maintenant les résultats de la deuxième année de culture intensive. Rappelons que cette étude a pour but d'apprécier la qualité physico-chimique des eaux de ruissellement et le drainage arrivant dans le milieu ostréicole par l'intermédiaire du Chenal de Brouage. Les compagnes des deux années précédentes après un point zéro et une première année de mise en culture avaient fait apparaître, outre la présence d'insecticides rémanents, une dominance qualitative et quantitative des herbicides aussi bien dans l'eau que dans la matière vivante. Chaque année, des difficultés techniques différentes limitent l'information recueillie,... |
Tipo: Text |
Palavras-chave: Qualité des eaux; Marais Maritime; Agriculture; Rejets agricoles; Chenal de Brouage; Charente-Maritime. |
Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/00087/19849/17499.pdf |
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Masson, Daniel; Faury, Nicole; Ratiskol, Jacqueline. |
Le bassin de Marennes Oléron, comme toutes les zones conchylicoles, est susceptible d'être touché par les apports pollués. Dans ce domaine, le vecteur potentiel que constitue la Charente méritait d'être étudié plus particulièrement, la qualité bactériologique des eaux pouvant affecter par la suite la salubrité des coquillages. L'étude précédente (Masson et al. 1988) a fait apparaître que la pollution bactérienne apportée par la Charente (particulièrement en étiage) sédimentait rapidement, la quantité de germes devenant très faible à la sortie de l'estuaire. Toutefois, comme l'enjeu économique est important (perspectives européennes) il était nécessaire de confirmer ce résultat avec un système de suivi identique à celui utilisé en 1987. De plus, un certain... |
Tipo: Text |
Palavras-chave: Bactériologie; Qualité eaux; Salubrité coquillages; Pollution bactérienne; Estuaire; Charente; Charente Maritime Dep.. |
Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/00087/19827/17469.pdf |
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Ropert, Michel; Lefebvre, Sebastien; Soletchnik, Patrick; Blin, J. L.; Faury, Nicole; Struski, Caroline. |
The "trophic risk" has been demonstrated in the bioenergetic model developed in vivo and based on various diet conditions similar to those of different types of oyster culture basins (eutrophic, heterotrophic and oligotrophic) [22, 23]. The trophic resource mortality relationship differs from one basin to another, according to the quantity of this resource. Multy-varied analysis (ACP) conducted on a total of 11 oyster culture sites and handled in a database matching mortality and environmental data, show that the trophic indicator "chlorophyll a" (often coupled with temperature) is involved in a positive correlation with the mortality of both age classes (1 and 2 years old). The stock transfer from the Bay of Veys to the west coast of Cotentin in spring... |
Tipo: Text |
Palavras-chave: MOREST; Crassostrea gigas; Physiologie; Phéopigments; Ressources trophiques; Modèle bioénergétique; Mortalités; Huîtres. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/acte-3410.pdf |
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Pauletto, Marianna; Segarra, Amelie; Montagnani, Caroline; Quillien, Virgile; Faury, Nicole; Le Grand, Jacqueline; Miner, Philippe; Petton, Bruno; Labreuche, Yannick; Fleury, Elodie; Fabioux, Caroline; Bargelloni, Luca; Renault, Tristan; Huvet, Arnaud. |
Double-stranded RNA (dsRNA)-mediated genetic interference (RNAi) is a widely used reverse genetic tool for determining the loss-of-function phenotype of a gene. Here, the possible induction of an immune response by long dsRNA was tested in a marine bivalve (Crassostrea gigas), as well as the specific role of the subunit 2 of the nuclear factor kappa B inhibitor (I kappa B2). This gene is a candidate of particular interest for functional investigations in the context of oyster mass mortality events, as Cg-I kappa B2 mRNA levels exhibited significant variation depending on the amount of ostreid herpesvirus 1 (OsHV-1) DNA detected. In the present study, dsRNAs targeting Cg-I kappa B2 and green fluorescent protein genes were injected in vivo into oysters... |
Tipo: Text |
Palavras-chave: Anti-viral response; RNA interference; Inhibitor of NF-kappa B; Marine bivalve; Ostreid herpesvirus 1. |
Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00409/52011/52698.pdf |
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Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan. |
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in... |
Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf |
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Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Razet, Daniel; Geairon, Philippe; Goulletquer, Philippe. |
The summer mortality patterns was studied in 1996 on the Ronce-les-Bains oyster bank (175 ha), located on the southern part of the Bay Marennes-Oleron (Atlantic coastline). Studies on growth, sexual maturation, survival rates and environmental variables were compared at fifteen experimental sites (on- and off-bottom culture). After 7 months, cumulative mortality reached 8-19 and 23-33% for off-bottom and on-bottom culture, respectively which is significantly higher for the latter case. A seasonal mortality trend was noted, with a significant increase related to air temperature greater than 20 degrees C and with a significant pre-spawning glycogen catabolism. Based on soft tissue flesh growth results from both types of culture, several empirical models were... |
Tipo: Text |
Palavras-chave: Atlantic Ocean; Crassostrea gigas; Mortality; Growth rates; Océan Atlantique; Crassostrea gigas; Mortalité; Croissance. |
Ano: 1999 |
URL: http://archimer.ifremer.fr/doc/1999/publication-862.pdf |
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Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Razet, Daniel; Geairon, Philippe; Robert, Stephane; Goulletquer, Philippe. |
The cupped oyster, Crassostrea gigas was first introduced in France in 1967 [13]. On the Atlantic coast, the Marennes Oléron Bay (Figure 1) is the first European oyster culture basin with a cupped oyster (Crassostrea gigas) stock of 110,000 tons and an annual output of 30,000 to 40,000 tons. The Ronce oyster bank, located at the south of the bay (Figure 1), is made of 1,600 concessions spread out over 175 ha. It produces 8,000 to 10,000 tons of cupped oysters each year. In Japan, Crassostrea gigas has been cultivated for more than 1,000 years (Farley, 1992). Since the beginning of the 1960's, oyster mortality has multiplied throughout the world (Imaï et al., 1965; Sinderman, 1976; Mackin, 1961; Beattie et al, 1980). In the 60's and 70's, Japan and the west... |
Tipo: Text |
Palavras-chave: Crassostrea gigas; Physiologie; Pontes; Maturation; Croissance; Marennes Oléron; Huîtres; Mortalités. |
Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/1998/rapport-3380.pdf |
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Soletchnik, Patrick; Le Moine, Olivier; Faury, Nicole; Razet, Daniel; Geairon, Philippe; Robert, Stephane; Goulletquer, Philippe; Taillade, Sylvie. |
The 1999 monitoring of the C. gigas mortality rates showed a 32 to 40% loss for the on-bottom rearings located on the Ronce-Perquis oyster bank. In 1997, the averaged mortality rate varied between 23 and 29% without significant site effect. These mortality rates were ranging from 17 to 32% in 1998 with significant limited mortality rate on site 4 (17%). However, those rates were underestimated by 7 to 8%, based on our 1999 study, Actually, the spatial distribution for these rates on 2 experimental sites divided into 25 subunits demonstrated a large variability ranging from 17-37% and 22-56% for sites 1 and 2, respectively. In spite this large variability, the mortality rates results were larger on the muddy bottom site. In contrast to site 1, the oysters... |
Tipo: Text |
Palavras-chave: Bay of Marennes Oleron; Crassostrea gigas; Reproductive effort; Biochemical composition; Growth; Sampling strategy; Mortality rates; Bassin de Marennes Oléron; Crassostrea gigas; Effort de reproduction; Composition biochimique; Croissance; Méthodologie d'estimation; Taux de mortalité. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/rapport-1641.pdf |
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Registros recuperados: 120 | |
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